Sisters of the Third Order of Saint Francis, then the Poor Clares, were present in Montelupone from 1567. The construction of a real convent began in 1592 and in 1747 the building was extended and then consecrated in 1801. Following changes to the interior, the white plastered single chamber was enlarged, raised and enriched with works of art. The Virgin with Baby Jesus and Saints John the Evangelist, Nicholas of Bari and Clare by Onofrio Gabriello were placed on the high altar, whilst the Poor Clares’ choir in the rear was designed by Andrea Vici and created in 1775 by G. Matteucci and I. Rossini. The sisters continued to own the building until 1903.